MKNK2

MAPK interacting serine/threonine kinase 2
TCGA Data Summary

These figures show a summary of data collected by the cancer genome atlas for MKNK2. The mutations heatmaps shows the fraction samples with each type of genetic mutation, while the copy number variation shows the percentage of samples where a deletion or amplication was dectected. Finally, the mRNA expression tab shows the amount of mRNA detected on a log-2 scale for each cancer type. The X-axis cancer type abbreviations are described here. This summary of the cancer genome atlas (TCGA) was collated from firebrowse developed by the Broad Institute. The code used to produce these figures is available through github.


PDB Kinase Domains

Kinase Domain Structure:

  • Title: Structure of human Mnk2 Kinase Domain mutant D228G
  • Resolution: 3.2

Associated Compounds:

  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Structure of human Mnk2 Kinase Domain
  • Resolution: 2.1

Associated Compounds:

  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Crystal Structure of Mnk2-D228G in complex with Inhibitor
  • Resolution: 3.05

Associated Compounds:

  • 5-[(7H-purin-6-yl)amino]-1H-isoindol-1-one
  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Crystal Structure of Mnk2-D228G in Complex With Inhibitor
  • Resolution: 3.38

Associated Compounds:

  • 3-(pyridin-4-yl)imidazo[1,2-b]pyridazine
  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Crystal Structure of Mnk2-D228G in Complex With Inhibitor
  • Resolution: 2.8

Associated Compounds:

  • 3-(pyridin-3-yl)imidazo[1,2-a]pyridine-8-carboxamide
  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Co-crytsal Structure of MNK2 in Complex With an Inhibitor
  • Resolution: 2.9

Associated Compounds:

  • CHLORIDE ION
  • (3R)-3-methyl-5-[(pyrimidin-4-yl)amino]-2,3-dihydro-1H-isoindol-1-one
  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Crystal Structure of Mnk2-D228G in complex with Staurosporine
  • Resolution: 2.71

Associated Compounds:

  • STAUROSPORINE
  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Co-crystal structure of MNK2 in Complex With Inhibitor
  • Resolution: 2.95

Associated Compounds:

  • 3,3-dimethyl-6-[(pyrimidin-4-yl)amino]-2,3-dihydroimidazo[1,5-a]pyridine-1,5-dione
  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Co-crystal structure of MNK2 in complex with an inhibitor
  • Resolution: 3.6

Associated Compounds:

  • 3-phenyl-5-(pyridin-4-yl)-1H-indazole
  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Crystal Structure of Mnk2-D228G in complex with Inhibitor
  • Resolution: 3.36

Associated Compounds:

  • 4-[(9H-purin-6-yl)amino]benzamide
  • ZINC ION
View This Structure on RCSB PDB

Kinase Domain Structure:

  • Title: Crystal Structure of Mnk2-D228G in complex with Inhibitor
  • Resolution: 3.32

Associated Compounds:

  • 6'-[(6-aminopyrimidin-4-yl)amino]-8'-methyl-2'H-spiro[cyclohexane-1,3'-imidazo[1,5-a]pyridine]-1',5'-dione
  • ZINC ION
View This Structure on RCSB PDB

IDG Compound

Compound Name: UNC-BE1-004

Chemical Name: N-(2-methoxyethyl)-4-((6-(pyridin-4-yl)quinazolin-2-yl)amino)benzamide

CHEBI: 143119

Smile String: O=C(C1=CC=C(NC2=NC3=CC=C(C4=CC=NC=C4)C=C3C=N2)C=C1)NCCOC

Chemical Formula: C23H21N5O2

Molecular Weight: 399.45

cLogP: 1.3218

Source: SGC-UNC

This compound is available through special request from the SGC-UNC.

Additional data concerning this compound can be found here.


Interaction Networks

INDRA (Integrated Network and Dynamical Reasoning Assembler) is an automated model assembly system drawing from natural language processing systems and structured databases. It collects mechanistic and causal assertions, represents them in a standardized form (INDRA Statements), and assembles them into various modeling formalisms including causal graphs and dynamical models. More information on this work can be found on Github. In this particular figure, several interaction-types are depicted; physical complexes (blue), phosphorylation (black), and general up- or downregulation (green and red, respectively). Biomacromolecules are represented as squares, small molecule as circles, and biological processes and diamonds. The thickness of each line reflects a confidence score, with thicker lines higher in confidence.


Available Assays, Plasmids, Cell Lines and Animal Models
BioID Plasmids
These plasmids are generated by Ben Major's lab and are available through Addgene. These lentiviral expression plasmids have been used to generate protein-proximity or protein-protein interaction networks using the miniTurbo biotin ligase-based proximity-dependent biotinylation or traditional immuno-purification approaches coupled with mass spectrometry. More information about these plasmids can be found at the following links:
NanoBRET Assay
A MKNK2 NanoBRET Assay has been developed and validated. NanoLuc®-fused MKNK2 is available from Promega by special request. Additional information about this assay is available here.
Horizon KO HAP1 Cell Lines
MKNK2 MKNK2
IMPC Mouse KO
MGI:894279

Kinase Tissue Expression Summary

The expression of kinases varies widely across the human tissues assayed by the GTEx project and the Human Proteome Map. To gain a better understanding of the kinase tissue distribution, we've created an application that describes and summarizes the expression of each dark kinase in the context of the rest of the kinome. This interactive window onto the full applications shows the data associated with MKNK2. The graph summarizes the expression of the kinase across all the organ systems.

The full application can further explored at the Kinase Expression Data Application.


Reactome Pathways

The Reactome Knowledgebase of Human Biological Pathways and Processes is a curated and peer-reviewed knowledgebase available online as an open access resource that can be freely used and distributed by all members of the biological research community. This view of the reactome database is focused on MKNK2 and displays the pathways associated with MKNK2.