Peptide Performance Parameters and Robust Linear Regression Analysis of Peptide Calibration (Reverse) Curves for the Dark Kinases
| Gene Symbol | Protein Name | Peptide Sequence | LOD (fmol)2 | LOQ (fmol)3 | Calibration Curve Equation |
|---|---|---|---|---|---|
| CDK12 | Cyclin Dependent Kinase 12 | DTGELVALK1 | 0.05 | 0.15 | y=1.01x-0.16 |
| CDK12 | Cyclin Dependent Kinase 12 | LCGSPCPAVWPDVIK | 2.4 | 7.2 | y=1.10x-1.28 |
| CDK13 | Cyclin Dependent Kinase 13 | ICGSPCPAVWPDVIK | 2.4 | 7.2 | y=1.106x-0.66 |
| CDK13 | Cyclin Dependent Kinase 13 | VENNLIVDK | 0.08 | 0.24 | y=1.03x-0.21 |
| PKMYT1 | Protein Kinase, Membrane Associated Tyrosine/Threonine 1 | ATAEALLALPVLR | 0.04 | 0.12 | y=1.09x-0.16 |
| PKMYT1 | Protein Kinase, Membrane Associated Tyrosine/Threonine 1 | GSFPSFEPR | 0.14 | 0.42 | y=1.01x-0.15 |
| PKMYT1 | Protein Kinase, Membrane Associated Tyrosine/Threonine 1 | SLPPPPPAK | 0.35 | 1.05 | y=1.13x-0.41 |
| TLK2 | Tousled Like Kinase 2 | AEPYETSQGK | 0.2 | 0.6 | y=1.36x-0.05 |
| TLK2 | Tousled Like Kinase 2 | ISALENSK | 0.17 | 0.51 | y=1.05x-0.30 |
| CSNK1G1 | Casein Kinase 1 Gamma 1 | LDFFEKPDYEYLR | 0.352 | 1.056 | y=1.24x+-0.251 |
| CSNK1G1 | Casein Kinase 1 Gamma 1 | TLFTDLFEK | 0.179 | 0.537 | y=1.21x+-0.536 |
| CSNK1G3 | Casein Kinase 1 Gamma 3 | DVKPENFLIGRPGNK | 0.101 | 0.303 | y=1.32x+-0.123 |
| CSNK1G3 | Casein Kinase 1 Gamma 3 | GTGSSSSGVLMVGPNFR | 0.0202 | 0.0606 | y=1.06x+-0.149 |
| DGKH | Diacylglycerol Kinase Eta | GAELLHLER | 0.51 | 1.53 | y=1.13x+-0.456 |
| DGKH | Diacylglycerol Kinase Eta | ILNSDEHAVVISSAK | 2.84 | 8.52 | y=1.14x+0.538 |
| ITPK1 | Inositol-Tetrakisphosphate 1-Kinase | LLAEPAGGLVGER | 0.0582 | 0.1746 | y=0.983x+-0.338 |
| ITPR3 | Inositol 1,4,5-Trisphosphate Receptor Type 3 | DYDSNLNASR | 0.0336 | 0.1008 | y=1.11x+-0.0823 |
| LMTK3 | Lemur Tyrosine Kinase 3 | GAGETLAGDPAEVLGER | 0.0177 | 0.0531 | y=0.868x+-0.0802 |
| LMTK3 | Lemur Tyrosine Kinase 3 | LFLDLGPPQGNSEQIK | 0.398 | 1.194 | y=1.08x+0.0648 |
| LRRK1 | Leucine Rich Repeat Kinase 1 | VTIYSFTGNQR | 12.1 | 36.3 | y=1.17x+-4.06 |
| LRRK1 | Leucine Rich Repeat Kinase 1 | YFIEASPLPSSYPGK | 0.198 | 0.594 | y=1.37x+-0.102 |
| MAP3K14 | Mitogen-Activated Protein Kinase Kinase Kinase 14 | EIPPSCAPLTAQAIQEGLR | 0.134 | 0.402 | y=1.04x+-1.24 |
| MAP3K14 | Mitogen-Activated Protein Kinase Kinase Kinase 14 | TEDNEGVLLTEK | 0.0985 | 0.2955 | y=1.2x+-0.221 |
| NRK | Nik Related Kinase | EEPQVQALQQLQGAAR | 0.0487 | 0.1461 | y=0.8x+-0.0778 |
| NRK | Nik Related Kinase | LLVDIHVPDGFK | 2.2 | 6.6 | y=0.839x+5.23 |
| PIK3C2B | Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Beta | ISDVFLCR | 1.02 | 3.06 | y=1.53x+-0.431 |
| PIK3C2B | Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Beta | LIESSLGSVATK | 0.075 | 0.225 | y=1.4x+-0.148 |
| PIP5K1A | Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha | LEVAESEFTH | 0.0942 | 0.2826 | y=1.06x+-0.138 |
| PIP5K1A | Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha | SVDSSGETTYK | 0.0581 | 0.1743 | y=1.23x+-0.127 |
| STK3 | Serine/Threonine Kinase 3 | LSEDSLTK | 2.65 | 7.95 | y=0.961x+0.00242 |
| STK3 | Serine/Threonine Kinase 3 | TLIEDEIATILK | 0.0572 | 0.1716 | y=1.11x+-0.213 |
| UCK1 | Uridine-Cytidine Kinase 1 | LFVDTDSDVR | 0.0094 | 0.0282 | y=0.962x+-0.0861 |
| UCK1 | Uridine-Cytidine Kinase 1 | TVEVPTYDFVTHSR | 0.168 | 0.504 | y=1.19x+-0.429 |
| UCK2 | Uridine-Cytidine Kinase 2 | IVQLLGQNEVDYR | 2.77 | 8.31 | y=0.55x+0.213 |
| UCK2 | Uridine-Cytidine Kinase 2 | LFVDTDADTR | 0.0598 | 0.1794 | y=1.13x+-0.102 |
| VRK2 | VRK Serine/Threonine Kinase 2 | AANLLLGYK | 0.213 | 0.639 | y=1.18x+-0.326 |
| VRK2 | VRK Serine/Threonine Kinase 2 | TNLLDELPQSVLK | NA | NA | y=NAx+NA |
| CAMK1D | Calcium/Calmodulin Dependent Protein Kinase ID | ESSIENEIAVLR | 4.62 | 13.86 | y=0.85x-3.18 |
| CAMK1D | Calcium/Calmodulin Dependent Protein Kinase ID | ETLGTGAFSEVVLAEEK | 0.3 | 0.9 | y=0.95x-0.14 |
| CDC42BPA | CDC42 Binding Protein Kinase Alpha | GPLGIDPQK | 0.04 | 0.12 | y=1.05x-0.1 |
| CDC42BPA | CDC42 Binding Protein Kinase Alpha | LFLYDIAEGK | 0.23 | 0.69 | y=1.12x-0.25 |
| CDC42BPB | CDC42 Binding Protein Kinase Beta | AILTAAIVDADR | 0.26 | 0.78 | y=1.15x+0.01 |
| CDC42BPB | CDC42 Binding Protein Kinase Beta | ELEAQLDDAVAEASK | 0.06 | 0.18 | y=1.08x-0.35 |
| MARK4 | Microtubule Affinity Regulating Kinase 4 | AENLLLDAEANIK | 0.42 | 1.26 | y=0.6x+0.04 |
| MARK4 | Microtubule Affinity Regulating Kinase 4 | FLVLNPAK | 0.22 | 0.66 | y=1.15x-0.22 |
| MAST2 | Microtubule Associated Serine/Threonine Kinase 2 | EDPALSITQVPDASGDR | 0.11 | 0.33 | y=0.87x-0.01 |
| MAST2 | Microtubule Associated Serine/Threonine Kinase 2 | VAISTTPLENTSIK | 0.07 | 0.21 | y=1.02x-0.18 |
| MAST3 | Microtubule Associated Serine/Threonine Kinase 3 | LQEFLTAYAPGAR | 0.47 | 1.41 | y=1.02x-0.05 |
| MAST3 | Microtubule Associated Serine/Threonine Kinase 3 | TDLPQYIIGQLGLAK | 0.09 | 0.27 | y=1.12x-0.07 |
| PHKG2 | Phosphorylase Kinase Catalytic Subunit Gamma 2 | LLQVDPEAR | 0.12 | 0.36 | y=0.93x-0.07 |
| PHKG2 | Phosphorylase Kinase Catalytic Subunit Gamma 2 | LSPEQLEEVR | 0.08 | 0.24 | y=1.24x-0.15 |
| RPS6KC1 | Ribosomal Protein S6 Kinase C1 | DLNPNNILLNDR | 0.18 | 0.54 | y=1.01x-0.09 |
| STK17A | Serine/Threonine Kinase 17a | FEEPLLQEIPGEFIY | 3.38 | 10.14 | y=11.98x-4.77 |
| STK17A | Serine/Threonine Kinase 17a | IVDFGLSR | 0.34 | 1.02 | y=1.2x-0.21 |
| STK32B | Serine/Threonine Kinase 32b | ALMPGFVPNK | 0.13 | 0.39 | y=1.02x-0.24 |
| STK32B | Serine/Threonine Kinase 32b | EEFIIFNR | 0.36 | 1.08 | y=1.02x-0.18 |
| STK32C | Serine/Threonine Kinase 32c | DAAEPVEDEAER | 0.03 | 0.09 | y=1.02x-0.12 |
| STK32C | Serine/Threonine Kinase 32c | EPLPAPESR | 0.24 | 0.72 | y=1.2x-0.1 |
| CDKL5 | Cyclin Dependent Kinase Like 5 | DIKPENLLISHNDVLK | 0.8276 | 2.483 | y=1.07x+0.32 |
| CDKL5 | Cyclin Dependent Kinase Like 5 | NLSEGNNANYTEYVATR | 0.3331 | 0.9992 | y=1.10x-0.59 |
| CDK14 | Cyclin Dependent Kinase 14 | LQEEEGTPFTAIR | 0.4306 | 1.292 | y=0.90x+0.14 |
| CDK14 | Cyclin Dependent Kinase 14 | LSYVNHAEDLASK | 0.143 | 0.429 | y=1.03x-0.22 |
| CDK16 | Cyclin Dependent Kinase 16 | LDSDGADLLTK | 1.203 | 3.609 | y=0.956x+0.95 |
| CDK16 | Cyclin Dependent Kinase 16 | LPDTTSIFALK | 10.04 | 30.11 | y=0.99x+3.06 |
| CDK17 | Cyclin Dependent Kinase 17 | FLQYESK | 0.524 | 1.572 | y=0.95x-0.55 |
| CDK17 | Cyclin Dependent Kinase 17 | IHALPESVSIFSLK | 0.1792 | 0.5376 | y=0.96x-0.05 |
| CDK18 | Cyclin Dependent Kinase 18 | LDTDGIHLLSSLLLYESK | 0.3689 | 1.107 | y=0.86x+0.11 |
| CDK18 | Cyclin Dependent Kinase 18 | LPQEFLQK | 0.1548 | 0.4645 | y=1.07x-0.28 |
| CDK10 | Cyclin Dependent Kinase 10 | FPWLSEAGLR | 0.8737 | 2.621 | y=1.12x-1.15 |
| CDK10 | Cyclin Dependent Kinase 10 | TADFGLAR | 0.1209 | 0.3627 | y=1.12x-0.15 |
| MAPK15 | Mitogen-Activated Protein Kinase 15 | APEVLLSSHR | 1.213 | 3.638 | y=1.13x+0.08 |
| MAPK15 | Mitogen-Activated Protein Kinase 15 | MFSTSALQGAQGGAR | 0.06565 | 0.1969 | y=1.19x-0.28 |
| MAPK15 | Mitogen-Activated Protein Kinase 15 | VASVQQVPPR | 2.749 | 8.246 | y=1.04x+0.28 |
| CDKL2 | Cyclin Dependent Kinase 2 | DIKPENILVSQSGVVK | 0.9162 | 2.749 | y=1.06x-0.43 |
| CDKL2 | Cyclin Dependent Kinase 2 | TLAAPGEVYTDYVATR | 4.251 | 12.75 | y=0.89x+4.96 |
| CDK15 | Cyclin Dependent Kinase 15 | DLKPQNLLISHLGELK | 0.8213 | 2.464 | y=0.99x+0.35 |
| CDK15 | Cyclin Dependent Kinase 15 | INGQLVALK | 0.3633 | 1.09 | y=1.03x-0.08 |
| CLK4 | CDC Like Kinase 4 | ITLDEALQHPFFDLLK | 0.2064 | 0.6193 | y=1.10x-0.16 |
| CLK4 | CDC Like Kinase 4 | SEIQVLEHLNSTDPNSVFR | 0.2591 | 0.7773 | y=1.04x+0.01 |
| ICK | Intestinal Cell Kinase | QHQASQPPLHLTYPYK | 0.4431 | 1.329 | y=0.96x+0.99 |
| ICK | Intestinal Cell Kinase | QLGDGTYGSVLLGR | 1.03 | 3.089 | y=1.17x+0.30 |
| CDK11A | Cyclin Dependent Kinase 11A | ELGTPSEK | 0.2053 | 0.6159 | y=0.95x-0.19 |
| CDK11A | Cyclin Dependent Kinase 11A | IYIVMNYVEHDLK | 0.4614 | 1.384 | y=1.05x+0.05 |
| DYRK1B | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B | ISPLGALQHGFFR | 0.06326 | 0.1898 | y=1.24x-0.13 |
| DYRK3 | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 3 | VVNPASAFQGLGSK | 1420 | 4260 | y=NAx+NA |
| DYRK3 | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 3 | VVPLTPEQALK | 0.1251 | 0.3753 | y=1.17x-0.15 |
| CSNK2A2 | Casein Kinase 2 Alpha 2 | VLGTEELYGYLK | 0.2696 | 0.8089 | y=1.01x-0.03 |
| CSNK2A2 | Casein Kinase 2 Alpha 2 | VYAEVNSLR | 0.01735 | 0.05206 | y=0.98x-0.07 |
| CDK11B | Cyclin Dependent Kinase 11B | AYTPVVVTLWYR | 0.04798 | 0.1439 | y=1.01x-0.07 |
| CDK11B | Cyclin Dependent Kinase 11B | IWPGYSELPAVK | 0.08251 | 0.2475 | y=0.95x-0.17 |
| CDK17 | Cyclin Dependent Kinase 17 | ASLSEIGFGK | 0.2069 | 0.6207 | y=0.94x-0.12 |
| CDK17 | Cyclin Dependent Kinase 17 | LDSEGIELITK | 0.8516 | 2.555 | y=1.36x-0.0006 |
| CDK18 | Cyclin Dependent Kinase 18 | ASLSDIGFGK | 3.753 | 11.26 | y=1.24x+0.42 |
| CDK18 | Cyclin Dependent Kinase 18 | LGEGTYATVFK | 155 | 465 | y=NAx+NA |
| PRPF4B | Pre-MRNA Processing Factor 4B | LDDLALLEDLEK | 0.3352 | 1.006 | y=0.81x+0.15 |
| PRPF4B | Pre-MRNA Processing Factor 4B | SINEENGEVSEDQSQNK | 1.995 | 5.985 | y=0.78x+0.56 |
| CDK10 | Cyclin Dependent Kinase 10 | DGIPISSLR | 0.01933 | 0.05799 | y=0.85x+0.15 |
| CDK10 | Cyclin Dependent Kinase 10 | DTQTDEIVALK | 0.07964 | 0.2389 | y=0.96x-0.20 |
| DYRK2 | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 2 | APEVILGAR | 0.08224 | 0.2467 | y=0.98x-0.12 |
| DYRK2 | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 2 | ITESTGAITSISK | 0.02801 | 0.08404 | y=1.21x-0.12 |
| CLK4 | CDC Like Kinase 4 | DIESGYR | 2.954 | 8.863 | y=1.13x-2.52 |
| CLK4 | CDC Like Kinase 4 | ESWGHESYR | 0.06478 | 0.1943 | y=0.94x-0.30 |
| CDK11A | Cyclin Dependent Kinase 11A | IWPGYSELPVVK | 0.1632 | 0.4897 | y=1.08x-0.22 |
| DYRK1B | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B | AFLNQAQIELR | 2.054 | 6.161 | y=0.71x+0.29 |
| DYRK1B | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B | LQEVLGVQTGGPGGR | 0.3524 | 1.057 | y=1.10x-0.10 |
| COQ8A | Coenzyme Q8A | AVLGSSPFLSEANAER | 0.02 | 0.06 | y=1.12x-0.23 |
| COQ8A | Coenzyme Q8A | VALLDFGATR | 0.04 | 0.12 | y=0.96x-0.03 |
| RIOK2 | RIO Kinase 2 | ESDIYIVANEEGQQFALK | 1.49 | 4.47 | y=0.84x+0.01 |
| RIOK2 | RIO Kinase 2 | FSYESELFPTFK | 0.14 | 0.42 | y=1.04x-0.17 |
| EEF2K | Eukaryotic Elongation Factor 2 Kinase | LTPQAFSHFTFER | 0.51 | 1.53 | y=1.04x-0.23 |
| EEF2K | Eukaryotic Elongation Factor 2 Kinase | YNAVTGEWLDDEVLIK | 0.89 | 2.67 | y=1.16x-0.15 |
| BCKDK | Branched Chain Ketoacid Dehydrogenase Kinase | DVVTLLAEGLR | 0.02 | 0.06 | y=1.13x-0.07 |
| BCKDK | Branched Chain Ketoacid Dehydrogenase Kinase | LTDFPPIK | 2.06 | 6.18 | y=1.21x-3.31 |
| ADCK5 | AarF Domain Containing Kinase 5 | AINVALGAPVDR | 0.04 | 0.12 | y=1.33x-0.25 |
| SCYL2 | SCY1 Like Pseudokinase 2 | LGSSSLTNIPEEVR | 0.54 | 1.62 | y=0.94x+0.1 |
| SCYL2 | SCY1 Like Pseudokinase 2 | LILPELGPVFK | 0.28 | 0.84 | y=1.1x+0.07 |
| PRAG1 | PEAK1 Related, Kinase-Activating Pseudokinase 1 | LAPEIVSASQYR | 0.09 | 0.27 | y=1.03x-0.01 |
| PRAG1 | PEAK1 Related, Kinase-Activating Pseudokinase 1 | LGGLYTQSLAR | 0.08 | 0.24 | y=1.01x-0.07 |
| SCYL3 | SCY1 Like Pseudokinase 3 | DYYNTLLQTGDPFSQPIK | 0.15 | 0.45 | y=0.98x+0.02 |
| SCYL3 | SCY1 Like Pseudokinase 3 | LVPLLLNQLVFAEPVAVK | 0.4 | 1.2 | y=0.86x+0.03 |
| NEK11 | NIMA Related Kinase 11 | IVEGDTPSLPER | 0.04 | 0.12 | y=1.03x-0.08 |
| NEK11 | NIMA Related Kinase 11 | LGSGSFGTVYLVSDK | 1.56 | 4.68 | y=0.7x+0.89 |
| NEK9 | NIMA Related Kinase 9 | GAFGEATLYR | 0.11 | 0.33 | y=1.07x+0.07 |
| NEK7 | NIMA Related Kinase 7 | AACLLDGVPVALK | 0.17 | 0.51 | y=1.1x-0.1 |
| NEK7 | NIMA Related Kinase 7 | GQFSEVYR | 0.16 | 0.48 | y=1.12x-0.03 |
| NEK7 | NIMA Related Kinase 7 | RPDVTYVYDVAK | 182.7 | 548 | y=NAx+NA |
| NEK7 | NIMA Related Kinase 7 | VQIFDLMDAK | 3.84 | 11.52 | y=1.18x-3.46 |
| TBCK | TBC1 Domain Containing Kinase | ALSPHNILLDR | 1.68 | 5.04 | y=0.96x-0.2 |
| TBCK | TBC1 Domain Containing Kinase | LFQSLDISER | 0.24 | 0.72 | y=1.12x-0.06 |
| POMK | Protein-O-Mannose Kinase | SQTPSERPTAQDVLETYQK | 0.47 | 1.41 | y=0.71x+0.09 |
| POMK | Protein-O-Mannose Kinase | VALSQLTSLEMK | 1.01 | 3.03 | y=1.1x+0.07 |
| STK36 | Serine/Threonine Kinase 36 | LGATPQESSLLAGILASELK | 0.92 | 2.76 | y=0.66x+0.98 |
| STK36 | Serine/Threonine Kinase 36 | SLQQEPGIHQVLVSLGASEK | 0.05 | 0.15 | y=0.57x-0.33 |
| NRBP2 | Nuclear Receptor Binding Protein 2 | LAAFLESTFLK | 0.1 | 0.3 | y=0.98x-0.09 |
| NRBP2 | Nuclear Receptor Binding Protein 2 | VIFITEYVSSGSLK | 0.15 | 0.45 | y=1.48x-0.22 |
- Emboldened amino acid indicates stable isotope labeled residue with either Lys -[13C6] [15N2] or Arg -[13C6] [15N4]. Cys residues are carbamidomethylated.
- The LOD was determined using a non-parametric method with eight LC-MS analyses without added analyte ((http://clinchem.aaccjnls.org/content/50/4/732).
- The LOQ was generated from the LOD (“Algorithms, Routines and S Functions for Robust Statistics” (CRC Press, Boca Raton, Florida, USA, 1993)).
Dark Kinome Kinases and their Signature Peptides
| Gene Symbol | Protein Name | Peptide Identifier | Peptide Sequence |
|---|---|---|---|
| CDK12 | Cyclin Dependent Kinase 12 | CDK12-DTG | DTGELVALK1 |
| CDK12 | Cyclin Dependent Kinase 12 | CDK12-LCG | LCGSPCPAVWPDVIK |
| CDK13 | Cyclin Dependent Kinase 13 | CDK13-ICG | ICGSPCPAVWPDVIK |
| CDK13 | Cyclin Dependent Kinase 13 | CDK13-VEN | VENNLIVDK |
| PKMYT1 | Protein Kinase, Membrane Associated Tyrosine/Threonine 1 | PKMYT1-ATA | ATAEALLALPVLR |
| PKMYT1 | Protein Kinase, Membrane Associated Tyrosine/Threonine 1 | PKMYT1-GSF | GSFPSFEPR |
| PKMYT1 | Protein Kinase, Membrane Associated Tyrosine/Threonine 1 | PKMYT1-SLP | SLPPPPPAK |
| TLK2 | Tousled Like Kinase 2 | TLK2-AEP | AEPYETSQGK |
| TLK2 | Tousled Like Kinase 2 | TLK2-ISA | ISALENSK |
| CSNK1G1 | Casein Kinase 1 Gamma 1 | CSNK1G1-LDF | LDFFEKPDYEYLR |
| CSNK1G1 | Casein Kinase 1 Gamma 1 | CSNK1G1-TLF | TLFTDLFEK |
| CSNK1G3 | Casein Kinase 1 Gamma 3 | CSNK1G3-DVK | DVKPENFLIGRPGNK |
| CSNK1G3 | Casein Kinase 1 Gamma 3 | CSNK1G3-GTG | GTGSSSSGVLMVGPNFR |
| DGKH | Diacylglycerol Kinase Eta | DGKH-GAE | GAELLHLER |
| DGKH | Diacylglycerol Kinase Eta | DGKH-ILN | ILNSDEHAVVISSAK |
| ITPK1 | Inositol-Tetrakisphosphate 1-Kinase | ITPK1-LLA | LLAEPAGGLVGER |
| ITPR3 | Inositol 1,4,5-Trisphosphate Receptor Type 3 | ITPR3-DYD | DYDSNLNASR |
| LMTK3 | Lemur Tyrosine Kinase 3 | LMTK3-GAG | GAGETLAGDPAEVLGER |
| LMTK3 | Lemur Tyrosine Kinase 3 | LMTK3-LFL | LFLDLGPPQGNSEQIK |
| LRRK1 | Leucine Rich Repeat Kinase 1 | LRRK1-VTI | VTIYSFTGNQR |
| LRRK1 | Leucine Rich Repeat Kinase 1 | LRRK1-YFI | YFIEASPLPSSYPGK |
| MAP3K14 | Mitogen-Activated Protein Kinase Kinase Kinase 14 | MAP3K14-EIP | EIPPSCAPLTAQAIQEGLR |
| MAP3K14 | Mitogen-Activated Protein Kinase Kinase Kinase 14 | MAP3K14-TED | TEDNEGVLLTEK |
| NRK | Nik Related Kinase | NRK-EEP | EEPQVQALQQLQGAAR |
| NRK | Nik Related Kinase | NRK-LLV | LLVDIHVPDGFK |
| PIK3C2B | Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Beta | PIK3C2B-ISD | ISDVFLCR |
| PIK3C2B | Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Beta | PIK3C2B-LIE | LIESSLGSVATK |
| PIP5K1A | Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha | PIP5K1A-LEV | LEVAESEFTH |
| PIP5K1A | Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha | PIP5K1A-SVD | SVDSSGETTYK |
| STK3 | Serine/Threonine Kinase 3 | STK3-LSE | LSEDSLTK |
| STK3 | Serine/Threonine Kinase 3 | STK3-TLI | TLIEDEIATILK |
| UCK1 | Uridine-Cytidine Kinase 1 | UCK1-LFV | LFVDTDSDVR |
| UCK1 | Uridine-Cytidine Kinase 1 | UCK1-TVE | TVEVPTYDFVTHSR |
| UCK2 | Uridine-Cytidine Kinase 2 | UCK2-IVQ | IVQLLGQNEVDYR |
| UCK2 | Uridine-Cytidine Kinase 2 | UCK2-LFV | LFVDTDADTR |
| VRK2 | VRK Serine/Threonine Kinase 2 | VRK2-AAN | AANLLLGYK |
| VRK2 | VRK Serine/Threonine Kinase 2 | VRK2-TNL | TNLLDELPQSVLK |
| CAMK1D | Calcium/Calmodulin Dependent Protein Kinase ID | CAMK1D-ESS | ESSIENEIAVLR |
| CAMK1D | Calcium/Calmodulin Dependent Protein Kinase ID | CAMK1D-ETL | ETLGTGAFSEVVLAEEK |
| CDC42BPA | CDC42 Binding Protein Kinase Alpha | CDC42BPA-GPL | GPLGIDPQK |
| CDC42BPA | CDC42 Binding Protein Kinase Alpha | CDC42BPA-LFL | LFLYDIAEGK |
| CDC42BPB | CDC42 Binding Protein Kinase Beta | CDC42BPB-AIL | AILTAAIVDADR |
| CDC42BPB | CDC42 Binding Protein Kinase Beta | CDC42BPB-ELE | ELEAQLDDAVAEASK |
| MARK4 | Microtubule Affinity Regulating Kinase 4 | MARK4-AEN | AENLLLDAEANIK |
| MARK4 | Microtubule Affinity Regulating Kinase 4 | MARK4-FLV | FLVLNPAK |
| MAST2 | Microtubule Associated Serine/Threonine Kinase 2 | MAST2-EDP | EDPALSITQVPDASGDR |
| MAST2 | Microtubule Associated Serine/Threonine Kinase 2 | MAST2-VAI | VAISTTPLENTSIK |
| MAST3 | Microtubule Associated Serine/Threonine Kinase 3 | MAST3-LQE | LQEFLTAYAPGAR |
| MAST3 | Microtubule Associated Serine/Threonine Kinase 3 | MAST3-TDL | TDLPQYIIGQLGLAK |
| PHKG2 | Phosphorylase Kinase Catalytic Subunit Gamma 2 | PHKG2-LLQ | LLQVDPEAR |
| PHKG2 | Phosphorylase Kinase Catalytic Subunit Gamma 2 | PHKG2-LSP | LSPEQLEEVR |
| RPS6KC1 | Ribosomal Protein S6 Kinase C1 | RPS6KC1-DLN | DLNPNNILLNDR |
| STK17A | Serine/Threonine Kinase 17a | STK17A-FEE | FEEPLLQEIPGEFIY |
| STK17A | Serine/Threonine Kinase 17a | STK17A-IVD | IVDFGLSR |
| STK32B | Serine/Threonine Kinase 32b | STK32B-ALM | ALMPGFVPNK |
| STK32B | Serine/Threonine Kinase 32b | STK32B-EEF | EEFIIFNR |
| STK32C | Serine/Threonine Kinase 32c | STK32C-DAA | DAAEPVEDEAER |
| STK32C | Serine/Threonine Kinase 32c | STK32C-EPL | EPLPAPESR |
| CDKL5 | Cyclin Dependent Kinase Like 5 | CDKL5-DIK | DIKPENLLISHNDVLK |
| CDKL5 | Cyclin Dependent Kinase Like 5 | CDKL5-NLS | NLSEGNNANYTEYVATR |
| CDK14 | Cyclin Dependent Kinase 14 | CDK14-LQE | LQEEEGTPFTAIR |
| CDK14 | Cyclin Dependent Kinase 14 | CDK14-LSY | LSYVNHAEDLASK |
| CDK16 | Cyclin Dependent Kinase 16 | CDK16-LDS | LDSDGADLLTK |
| CDK16 | Cyclin Dependent Kinase 16 | CDK16-LPD | LPDTTSIFALK |
| CDK17 | Cyclin Dependent Kinase 17 | CDK17-FLQ | FLQYESK |
| CDK17 | Cyclin Dependent Kinase 17 | CDK17-IHA | IHALPESVSIFSLK |
| CDK18 | Cyclin Dependent Kinase 18 | CDK18-LDT | LDTDGIHLLSSLLLYESK |
| CDK18 | Cyclin Dependent Kinase 18 | CDK18-LPQ | LPQEFLQK |
| CDK10 | Cyclin Dependent Kinase 10 | CDK10-FPW | FPWLSEAGLR |
| CDK10 | Cyclin Dependent Kinase 10 | CDK10-TAD | TADFGLAR |
| MAPK15 | Mitogen-Activated Protein Kinase 15 | MAPK15-APE | APEVLLSSHR |
| MAPK15 | Mitogen-Activated Protein Kinase 15 | MAPK15-MFS | MFSTSALQGAQGGAR |
| MAPK15 | Mitogen-Activated Protein Kinase 15 | MAPK15-VAS | VASVQQVPPR |
| CDKL2 | Cyclin Dependent Kinase 2 | CDKL2-DIK | DIKPENILVSQSGVVK |
| CDKL2 | Cyclin Dependent Kinase 2 | CDKL2-TLA | TLAAPGEVYTDYVATR |
| CDK15 | Cyclin Dependent Kinase 15 | CDK15-DLK | DLKPQNLLISHLGELK |
| CDK15 | Cyclin Dependent Kinase 15 | CDK15-ING | INGQLVALK |
| CLK4 | CDC Like Kinase 4 | CLK4-ITL | ITLDEALQHPFFDLLK |
| CLK4 | CDC Like Kinase 4 | CLK4-SEI | SEIQVLEHLNSTDPNSVFR |
| ICK | Intestinal Cell Kinase | ICK-QHQ | QHQASQPPLHLTYPYK |
| ICK | Intestinal Cell Kinase | ICK-QLG | QLGDGTYGSVLLGR |
| CDK11A | Cyclin Dependent Kinase 11A | CDK11A-ELG | ELGTPSEK |
| CDK11A | Cyclin Dependent Kinase 11A | CDK11A-IYI | IYIVMNYVEHDLK |
| DYRK1B | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B | DYRK1B-ISP | ISPLGALQHGFFR |
| DYRK3 | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 3 | DYRK3-VVN | VVNPASAFQGLGSK |
| DYRK3 | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 3 | DYRK3-VVP | VVPLTPEQALK |
| CSNK2A2 | Casein Kinase 2 Alpha 2 | CSNK2A2-VLG | VLGTEELYGYLK |
| CSNK2A2 | Casein Kinase 2 Alpha 2 | CSNK2A2-VYA | VYAEVNSLR |
| CDK11B | Cyclin Dependent Kinase 11B | CDK11B-AYT | AYTPVVVTLWYR |
| CDK11B | Cyclin Dependent Kinase 11B | CDK11B-IWP | IWPGYSELPAVK |
| CDK17 | Cyclin Dependent Kinase 17 | CDK17-ASL | ASLSEIGFGK |
| CDK17 | Cyclin Dependent Kinase 17 | CDK17-LDS | LDSEGIELITK |
| CDK18 | Cyclin Dependent Kinase 18 | CDK18-ASL | ASLSDIGFGK |
| CDK18 | Cyclin Dependent Kinase 18 | CDK18-LGE | LGEGTYATVFK |
| PRPF4B | Pre-MRNA Processing Factor 4B | PRPF4B-LDD | LDDLALLEDLEK |
| PRPF4B | Pre-MRNA Processing Factor 4B | PRPF4B-SIN | SINEENGEVSEDQSQNK |
| CDK10 | Cyclin Dependent Kinase 10 | CDK10-DGI | DGIPISSLR |
| CDK10 | Cyclin Dependent Kinase 10 | CDK10-DTQ | DTQTDEIVALK |
| DYRK2 | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 2 | DYRK2-APE | APEVILGAR |
| DYRK2 | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 2 | DYRK2-ITE | ITESTGAITSISK |
| CLK4 | CDC Like Kinase 4 | CLK4-DIE | DIESGYR |
| CLK4 | CDC Like Kinase 4 | CLK4-ESW | ESWGHESYR |
| CDK11A | Cyclin Dependent Kinase 11A | CDK11A-IWP | IWPGYSELPVVK |
| DYRK1B | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B | DYRK1B-AFL | AFLNQAQIELR |
| DYRK1B | Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B | DYRK1B-LQE | LQEVLGVQTGGPGGR |
| COQ8A | Coenzyme Q8A | COQ8A-AVL | AVLGSSPFLSEANAER |
| COQ8A | Coenzyme Q8A | COQ8A-VAL | VALLDFGATR |
| RIOK2 | RIO Kinase 2 | RIOK2 -ESD | ESDIYIVANEEGQQFALK |
| RIOK2 | RIO Kinase 2 | RIOK2 -FSY | FSYESELFPTFK |
| EEF2K | Eukaryotic Elongation Factor 2 Kinase | EEF2K-LTP | LTPQAFSHFTFER |
| EEF2K | Eukaryotic Elongation Factor 2 Kinase | EEF2K-YNA | YNAVTGEWLDDEVLIK |
| BCKDK | Branched Chain Ketoacid Dehydrogenase Kinase | BCKDK-DVV | DVVTLLAEGLR |
| BCKDK | Branched Chain Ketoacid Dehydrogenase Kinase | BCKDK-LTD | LTDFPPIK |
| ADCK5 | AarF Domain Containing Kinase 5 | ADCK5-AIN | AINVALGAPVDR |
| SCYL2 | SCY1 Like Pseudokinase 2 | SCYL2-LGS | LGSSSLTNIPEEVR |
| SCYL2 | SCY1 Like Pseudokinase 2 | SCYL2-LIL | LILPELGPVFK |
| PRAG1 | PEAK1 Related, Kinase-Activating Pseudokinase 1 | PRAG1-LAP | LAPEIVSASQYR |
| PRAG1 | PEAK1 Related, Kinase-Activating Pseudokinase 1 | PRAG1-LGG | LGGLYTQSLAR |
| SCYL3 | SCY1 Like Pseudokinase 3 | SCYL3-DYY | DYYNTLLQTGDPFSQPIK |
| SCYL3 | SCY1 Like Pseudokinase 3 | SCYL3-LVP | LVPLLLNQLVFAEPVAVK |
| NEK11 | NIMA Related Kinase 11 | NEK11-IVE | IVEGDTPSLPER |
| NEK11 | NIMA Related Kinase 11 | NEK11-LGS | LGSGSFGTVYLVSDK |
| NEK9 | NIMA Related Kinase 9 | NEK9-GAF | GAFGEATLYR |
| NEK7 | NIMA Related Kinase 7 | NEK7-AAC | AACLLDGVPVALK |
| NEK7 | NIMA Related Kinase 7 | NEK7-GQF | GQFSEVYR |
| NEK7 | NIMA Related Kinase 7 | NEK7-RPD | RPDVTYVYDVAK |
| NEK7 | NIMA Related Kinase 7 | NEK7-VQI | VQIFDLMDAK |
| TBCK | TBC1 Domain Containing Kinase | TBCK-ALS | ALSPHNILLDR |
| TBCK | TBC1 Domain Containing Kinase | TBCK-LFQ | LFQSLDISER |
| POMK | Protein-O-Mannose Kinase | POMK-SQT | SQTPSERPTAQDVLETYQK |
| POMK | Protein-O-Mannose Kinase | POMK-VAL | VALSQLTSLEMK |
| STK36 | Serine/Threonine Kinase 36 | STK36-LGA | LGATPQESSLLAGILASELK |
| STK36 | Serine/Threonine Kinase 36 | STK36-SLQ | SLQQEPGIHQVLVSLGASEK |
| NRBP2 | Nuclear Receptor Binding Protein 2 | NRBP2-LAA | LAAFLESTFLK |
| NRBP2 | Nuclear Receptor Binding Protein 2 | NRBP2-VIF | VIFITEYVSSGSLK |
- Emboldened amino acid indicates stable isotope labeled residue with either Lys -[13C6] [15N2] or Arg -[13C6] [15N4]. Cys residues are carbamidomethylated.